Solution CD2 (60 ml)，47016-2，Qiagen，凯杰

T4 RNA Ligase 2 catalyzes phosphodiester bond formation between a 5ʹ phosphate and 3ʹ hydroxyl of RNA. The preferred substrate is nicked double-stranded RNA but single-stranded RNA can also serve as a substrate. Ligation of single-stranded RNA substrates generates either intramolecular or intermolecular products. Besides nicked double-stranded RNA substrates, other nicked nucleic acids hybrids can be sealed. The strand containing the 5ʹ phosphate can either be DNA or RNA. The non-ligated strand of the duplex can be either RNA or DNA. T4 RNA ligase 2 requires ATP for activity unless the substrate is preadenylated on the 5ʹ end. A truncated version of T4 RNA ligase 2 is a better enzyme for preadenylated substrates because it generates less side-reaction ligation products than the full-length enzyme. T4 RNA Ligase 2 truncated catalyzes phosphodiester bond formation between a pre-adenylated 5′ phosphate (DNA or RNA) and the 3′ hydroxyl of RNA. The truncated enzyme contains the first 249 amino acids which makes the enzyme require a pre-adenylated 5′ terminal donor and eliminates the need for ATP. Because T4 RNA ligase 2 truncated cannot use the 5′ phosphate of RNA or DNA as a donor in the ligation reaction, it is useful for certain applications, such as linker ligations with pre-adenylated 5′ DNA to 3′ hydroxyl RNA. The desired specific ligation products are enhanced dramatically over unwanted background ligation products, making the truncated enzyme superior to the full-length enzyme for this use. Our T4 RNA Ligase 2 Truncated demonstrates equivalent or superior volume/volume performance when compared to analogous T4 RNA Ligase 2 Truncated products on the market. The full length and truncated T4 RNA Ligase 2 are supplied in 10 mM Tris-HCl, 100 mM NaCl, 0.1 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 7.5 at 25°C. T4 RNA Ligase 2 is supplied with 10X Ligation Buffer (cat. no. B6030), which contains the following: 500 mM Tris-HCl, 100 mM MgCl2, 50 mM DTT and 10 mM ATP; pH 7.6 at 25°C. T4 RNA Ligase 2 Truncated is supplied with 10X T4 RNA Ligase 2, Truncated Buffer (cat. no. B6070), which contains the following: 500 mM Tris-HCl, 100 mM MgCl2 and 50 mM DTT; pH 7.6 at 25°C.

• T4 RNA Ligase 2 ligates nicks on double- or single-stranded RNA
• T4 RNA Ligase 2 ligates 5ʹ phosphate and 3ʹ hydroxyl of RNA
• T4 RNA Ligase 2 Truncated facilitates linker ligations with pre-adenylated 5' DNA to 3' hydroxyl RNA
• T4 RNA Ligase 2 Truncated does not require ATP

This OEM by QIAGEN product is available for bulk purchase for the following commercial assay applications.

• Nick ligation in double-stranded RNA
• Ligate the 3' OH of RNA to the 5' phosphate of DNA in a double-stranded-format NGS RNA library construction (miRNA-seq or directional mRNA-seq)