RNeasy Micro Kit (50)，74004，Qiagen，凯杰

销售价： ¥ 8150.00 / 件

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订货号： 7KD9241

品牌型号： Qiagen 74004

货期： 45个工作日

最小订货量： 1件

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商品介绍
规格参数
包装参数

产品介绍 Product Description

RNeasy Kits are the gold standard for total RNA isolation. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica membrane RNeasy spin columns or 96-well plates. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured cell samples using silica membrane RNeasy 96 plates. A dedicated RNeasy QIAcube Kit enables automated purification of 1–12 samples on the QIAcube Connect.

The QIAwave RNA Mini Kit is an eco-friendlier version of our standard RNeasy Mini Kit. Reducing plastic and cardboard usage by up to 60% and 57%, respectively, this version incorporates waste tubes made from 100% post-consumer recycled plastic that can be reused during the procedure. The QIAwave buffers are provided as concentrates, decreasing plastic use by up to 90% per bottle. Despite the visual differences, the QIAwave Kit remains user-friendly, with chemistry and performance identical to the standard kit's.

In partnership with My Green Lab, we've also assessed the environmental impact of the RNeasy Mini Kit and the QIAwave RNA Mini Kit. My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. These include:

Manufacturing
Responsible chemical management
Sustainable content within products and packaging materials
Disposal of the packaging at the end of life

产品特点 Features

Fast procedure delivering high-quality total RNA in minutes
Ready-to-use RNA for high performance in any downstream application
Consistent RNA yields from small to large amounts of starting material
No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
High-throughput processing in 96-well format and automatable protocols

技术参数 Specifications

Comparison of RNeasy and QIAwave RNA Kits

Features	RNeasy Micro Kit	RNeasy Mini Kit/QIAwave RNA Mini Kit	RNeasy Mini QIAcube Kit	RNeasy Midi Kit	RNeasy Maxi Kit	RNeasy 96 Kit
Applications	RNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, Microarray analysis	RNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, Microarray analysis	RNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, Microarray analysis	RNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, Microarray analysis	RNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, Microarray analysis	RNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, Microarray analysis
Elution volume	10–14 µl	30–100 µl	30–100 µl	300–500 µl	800–2400 µl	45–140 µl
Format	Spin column	Spin column	Spin column, preloaded into rotor adapters	Spin column	Spin column	96-well plate
Main sample type	Tissue, cells	Tissue, cells, yeast	Tissue, cells, yeast	Tissue, cells, yeast	Tissue, cells, yeast	Cultured cells
Processing	Manual (centrifugation)	Manual (centrifugation) or automated (QIAcube Connect)	Automated (QIAcube Connect)	Manual (centrifugation)	Manual (centrifugation)	Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Total RNA	Total RNA	Total RNA	Total RNA	Total RNA	Total RNA
Sample amount	<5 mg tissue, <5 x 105 cells	0.5–30 mg tissue, 10 to 1 x 107 animal or human cells	0.5–30 mg tissue, 1 x 107 cells	20–250 mg tissue, 5 x 106 to 1 x 108 animal or human cells	150 mg – 1 g tissue, Up to 5 x 108 animal or human cells	5 x 105
Technology	Silica technology	Silica technology	Silica technology	Silica technology	Silica technology	Silica technology
Time per run or per prep	30–40 minutes	20 minutes	–	<1 hour	1 hour	30–40 minutes
Yield	Varies	Varies	Varies	Varies	Varies	1.3–3.1 µg

产品应用 Applications

RNA purified with RNeasy and QIAwave RNA technology has A260/280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5) and is ideal for use in all applications. Downstream applications include: