QIAamp PowerFecal Pro DNA Kit (50)，51804，Qiagen，凯杰

QIAamp PowerFecal Pro DNA Kit

For the isolation of microbial DNA from stool and gut samples

Efficient lysis of bacteria and fungi from stool and gut samples

Up to 20-fold more DNA compared to alternative methods

Yield higher alpha diversity in sequencing compared to other methods

Recovery of inhibitor-free DNA for direct use in next-generation sequencing applications

Automate workflows on the QIAcube Connect

Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.

DNA was isolated from stool samples (200 mg) from three donors using commercially available sample preparation solutions and the new QIAamp PowerFecal Pro DNA Kit and compared. Yields were measured by fluorometric quantification (Qubit™).

The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high-quality DNA.

A DNA from duplicate 1.8 ml C. albicans cultures was isolated with the QIAamp PowerFecal DNA (A) or the QIAamp PowerFecal Pro DNA (B) Kits. B, C DNA from stool samples (200 mg) from three donors was prepared with the first-generation QIAamp PowerFecal DNA Kit B or the new QIAamp PowerFecal Pro DNA Kit C. DNA was analyzed via gel electrophoresis.

Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.

Stool samples (200 mg) were prepared using commercially available sample preparation solutions or the new QIAamp PowerFecal Pro DNA Kit and compared.

DNA purity was measured via UV spectrophotometry and the comparisons of A260/A280 A and A260/A230 B ratios are shown.

Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.

DNA prepared from stool samples was isolated with different methods and enriched. Analysis of the 16S rRNA genes was done using the QIAseq® 1-Step Amplicon Kit and data analysis was done using the Microbial Genomics Pro DNA Suite (CLC workbench). Alpha diversity was determined by total number of bacterial OTUs.

Extracting microbial DNA from stool and gut samples can be challenging. Researchers will isolate higher yields of pure microbial DNA from stool and gut samples more efficiently with our new QIAamp PowerFecal Pro DNA Kit.

The QIAamp Power Fecal Pro DNA Kit builds and improves on our original PowerFecal technology utilizing a novel bead tube and optimized chemistry for more efficient lysis of bacteria and fungi. Inhibitor removal has been streamlined, allowing researchers to eliminate challenging inhibitors from stool and gut samples faster. This results in total DNA that reveals higher alpha diversity through observed operational taxonomic units (OTUs) in downstream testing.

Isolation of DNA using the QIAamp PowerFecal Pro DNA Kit can be automated on the QIAcube Connect.

Want to try this solution for the first time? Request a quote for a trial kit.

Performance

Using improved technologies, the new QIAamp PowerFecal Pro Kit allows researchers to obtain higher yields of DNA from stool and gut samples compared with alternative methods (See: 'Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.').

Improved yields of higher quality DNA are also achieved when comparing the QIAamp PowerFecal Pro DNA Kit to the first generation QIAamp PowerFecal DNA Kit when running stool and fungal samples (See: 'The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high quality DNA.').

Principle

The streamlined Inhibitor Removal Technology® (IRT) used by the new QIAamp PowerFecal Pro DNA Kit is highly efficient at removing inhibitors while still decreasing sample processing time. The QIAamp PowerFecal Pro DNA Kits also showed the highest A260/A280 ratios, near 1.8, of any other commercially available kit, indicating the absence of inhibitors. Less variability was also observed between the samples processed with QIAamp PowerFecal Pro DNA Kits (See: ‘Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.’). Researchers using the QIAamp PowerFecal Pro DNA Kit can identify a greater bacterial diversity with increased OTUs from stool samples compared with the first-generation QIAamp PowerFecal Kit and other commercially available kits (See: ‘Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.’).

Procedure

Samples processed with the QIAamp PowerFecal Pro DNA Kit are added to a bead beating tube. Rapid and thorough

homogenization occurs using mechanical and chemical methods. Once cells are lysed, our patented IRT is used to remove

inhibitors. Total genomic DNA is captured on a silica membrane in a spin-column format. DNA is then washed and eluted,

ready for downstream applications.