Rneasy Plus 96 Kit (12)，74192，Qiagen，凯杰

RNeasy Plus Kits are next generation QIAGEN RNA extraction kits, combining high-quality RNA purification with effective gDNA removal with unique gDNA Eliminator columns or plates. Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues. The RNeasy Plus Micro Kit is specially designed for limited amounts of samples and isolates up to 45 µg pure total RNA The RNeasy Plus Mini Kit purifies up to 100 µg total RNA (>200 nt) from a single extraction with efficient gDNA removal. Both kits can be automated on the QIAcube Connect and samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system (available separately). The RNeasy Plus 96 Kit is for high-throughput purification of total RNA from cultured cells grown in 96-well plates.  In partnership with My Green Lab, we've also assessed the environmental impact of the RNeasy Mini Kit (50/250) and the QIAwave RNA Plus Mini Kit (50/250). My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. These include:  Manufacturing Responsible chemical management  Sustainable content within products and packaging materials  Disposal of the packaging at the end of life Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact (see figures "RNeasy Plus Mini Kit ACT environmental impact factor label  US ( 50 / 250 ),  EU ( 50 / 250 ) and UK ( 50 / 250 )" and “QIAwave RNA Plus Mini Kit ACT environment impact factor label  US ( 50 / 250 ), EU ( 50 / 250 ) and UK ( 50 / 250 )“.

• Efficient gDNA removal with unique gDNA Eliminator columns or plates (no need for DNase)
• High-quality total RNA in minutes using fast and simple extraction protocols
• Phenol-free RNA isolation
• High-throughput processing in 96-well format
• Ideal for sensitive applications such as real-time RT-PCR and RNA-seq

RNA purified using RNeasy Plus Kits is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as RNA-seq and quantitative real-time RT-PCR. The purified RNA can also be used in other applications.

The RNeasy Plus Micro Kit is suitable for small samples, including:

• Laser-microdissected cryosections
• Fine-needle aspirates

The ease with which RNA is purified in 96-well format makes the RNeasy Plus 96 Kit well suited for high-throughput gene expression analysis in areas such as:

• Drug discovery
• Biomedical research