QIAseq FastSelect RNA Lib Fish Kit (24)，334272，Qiagen，凯杰

QIAseq FastSelect RNA Library Kits utilize a simple and fast workflow for complete transcriptomics starting from 1 ng of total RNA. The simple workflow utilizes the FastSelect RNA removal reagents to suppress ribosomal RNA and other unwanted RNAs to enable gene expression and transcript identification from a variety of model systems including human, mouse, rat and related species, plants, yeast, zebrafish, flies, worms and bacteria. Researchers can also utilize mRNA enrichment kits (sold separately) and focus RNA-seq library construction on protein coding mRNAs. When used with the QIAseq UX Index IL UDI Index Kits (sold separately), 768 individual samples can be combined together for a single sequencing flow cell lane. The complete solution includes online access to the RNA-seq analysis portal (for supported species), which allows researchers to start with FASTQ files and perform read alignment, differential gene expression and pathway analysis. Furthermore, qPCR and digital PCR assays for biological verification can be easily identified in QIAGEN GeneGlobe following data analysis. QIAseq FastSelect RNA Library Kits are also supported with on-site software through QIAGEN’s CLC Genomics Workbench (sold separately). The final RNA-seq libraries created using the QIAseq FastSelect RNA Library Kits retain strand and directionality of the original transcript, increasing the accuracy of the final mapped transcripts and quantification.

• Start with 1–1000 ng of total RNA or enriched mRNA
• Integrated QIAseq FastSelect RNA removal reagent suppresses ribosomal RNA or other unwanted RNAs
• Sample barcoding following reverse transcription ensures no sample mix-ups
• Includes protocols for library construction for whole transcriptome or 3’ RNA-seq starting with total RNA or enriched mRNA
• Includes access to online RNA-seq read mapping, differential gene expression and pathway analysis using the RNA-seq Analysis Portal for supported species

QIAseq FastSelect RNA Library Kits support stranded RNA-seq library construction for transcript identification, fusion gene discovery and high-throughput gene expression. Input RNA can be high quality or fragmented, allowing for RNA-seq libraries from tissues, exosomes, FFPE samples and cell lines. RNA from eukaryotic, prokaryotic and fungi samples is also supported.