CD4+ CD25+ Reg. T Cell Iso. Kit, human，130-091-301，美天旎

The CD4⁺CD25⁺ Regulatory T Cell Isolation Kit was developed for the isolation of CD4⁺CD25⁺ cells from human PBMCs. The isolation is performed in a two-step procedure.

Regulatory CD4⁺CD25⁺ T cells are suppressor cells that neutralize other immune cells by various mechanisms. Their characteristic marker is the transcription factor FoxP3. Regulatory CD4⁺CD25⁺ T cells seem to suppress harmful immunological reactions to self or foreign antigens.

The isolation is performed in a two-step procedure. First, non-CD4⁺ T cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies against CD8, CD14, CD15, CD16, CD19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A) and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted over a MACS® Column. In the second step, the flow-through fraction of pre-enriched CD4⁺ T cells is labeled with CD25 MicroBeads for subsequent positive selection of CD4⁺CD25⁺ regulatory T cells.

The CD4⁺CD25⁺ Regulatory T Cell Isolation Kit has been used to investigate the role of CD4⁺CD25⁺ regulatory T cells in autoimmune diseases^1,2, antitumor immunity^3–6, allergy⁷, and in infectious diseases⁸. For further characterization, CD4⁺CD25⁺ regulatory T cells were isolated from patients suffering from rheumatoid arthritis¹ or multiple sclerosis², from acute T cell lymphoma patients⁴, from tumor biopsy specimens of non-Hodgkin lymphoma patients⁵, after dendritic cell vaccination⁹ as well as from patients with atopic dermatitis⁷.

For the first magnetic separation (depletion): LD or autoMACS^® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.