Anti-SARS-CoV-2 Nucleocapsid (NP) Human IgA ELISA Kit,96T,AK575028-96T,Abinscience

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 Nucleocapsid (NP) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 Nucleocapsid (NP) Human IgA present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgA antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 Nucleocapsid (NP) Human IgA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Stability and Storage	When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
Detection method	Colorimetric
Sample type	Plasma, Serum
Assay type	Quantitative
Sensitivity	0.5 ng/mL
Range	0.625 - 40 ng/mL
Recovery	80-120%
Background
Alternative Names
Shipping	2-8 ℃
Specifications	Nucleocapsid
Note	For Research Use Only.