Anti-Dupilumab Neutralizing Antibody ELISA kit,96T,AB732518-96T,Abinscience

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Dupilumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD124 antigen and then pipetted into the wells. Anti-Dupilumab Neutralizing Antibody in the sample competitively binds to Dupilumab with the biotin-labeled Human CD124 antigen. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Dupilumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.

Stability and Storage	When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
Detection method	Colorimetric
Sample type	Plasma, Serum
Assay type	Quantitative
Sensitivity	82.6 ng/mL
Range	156.25 - 10,000 ng/mL
Recovery	80-120%
Background
Alternative Names	CD124,REGN668,SAR231893, CAS: 1190264-60-8
Shipping	2-8 ℃
Specifications	Dupilumab
Note	For Research Use Only.