- 商品介绍
- 规格参数
- 包装参数
The miRNeasy Tissue/Cells Advanced Kits are the second generation of the well-established miRNeasy Kits. These improved versions combine a phenol-free advanced chemistry with gDNA Eliminator Columns or gDNA 96 Eliminator Plates for safe and convenient RNA isolation. They enable purification of total RNA, including small RNAs ≥18 nucleotides, from animal tissues and cells, including small samples. The kits use spin columns for low-throughput RNA purification and can be automated on the QIAcube Connect.
Phenol-free protocol – no need for phase separation or working under a hood
Fast and convenient procedure due to the advanced chemistry and gDNA Eliminator Columns
Efficient enrichment of miRNA and RNAs <200 nucleotides
Consistent, pure RNA for all downstream applications
Features | miRNeasy Tissue/Cells Advanced Micro | miRNeasy Tissue/Cells Advanced Mini | miRNeasy 96 Tissue/Cells Advanced Kit |
Applications | NGS, real-time RT-PCR, microarray | NGS, real-time RT-PCR, microarray | NGS, real-time RT-PCR, microarray |
Elution volume | Default 20 µl, min. 10 µl | 30 µl | 70-100 µl, min. 45 µl |
Format | Spin column | Spin column | RNeasy 96 Plate |
Main sample type | Tissue, cells | Tissue, cells | Tissue, cells |
Processing | Manual (centrifugation), automated (QIAcube Connect) | Manual (centrifugation), automated (QIAcube Connect) | Manual (centrifugation or centrifugation/vacuum), automated (QIAcube HT) |
RNA type | miRNA, total RNA | miRNA, total RNA | miRNA, total RNA |
Sample amount | Up to 5 mg tissue, up to 1 x 106 cells | Up to 30 mg frozen tissue (15 mg stabilized) or up to 1 x 107 cells | Up to 30 mg frozen tissue (15 mg stabilized) or up to 1 x 107 cells |
The miRNeasy Tissue/Cells Advanced Kits allow purification of miRNA and total RNA for a variety of applications, including:
• RNA-seq
• Quantitative, real-time RT-PCR
• Microarray analysis
• Northern blot analysis
长度(mm) | |
宽度(mm) | |
高度(mm) | |
重量(kg) |