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ACCUPRIME TAQ HIFI 1000 RXNS,12346094,Invitrogen

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订货号 9ZJ0589
品牌型号 Invitrogen 12346094
货期 询货期
最小订货量 1套
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  • 规格参数
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产品介绍 Product Description
规格
Exonuclease Activity: 3' - 5', 5' - 3'
Fidelity (vs. Taq): 9 X
GC-Rich PCR Performance: Low
Hot Start: Built-In Hot Start
Overhang: Mixed, 3'-A
Polymerase: AccuPrime™ Taq DNA Polymerase High Fidelity
Product Line: AccuPrime™
Quantity: 1000 reactions
Reaction Format: Separate Components
Reaction Speed: Standard
Shipping Condition: Dry Ice
Size (Final Product): 20 kb or less
储存
• 1 x 200 μL AccuPrime Taq DNA Polymerase, High Fidelity (5 U/μL)
• 4 × 1.25 mL 10X AccuPrime PCR Buffer I
• 4 × 1.25 mL 10X AccuPrime PCR Buffer II
• 1 x 1 mL 50 mM MgSO4

Store at -10°C to -30°C.
描述

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates. It includes an enzyme blend of Platinum Taq DNA Polymerase and a proofreading enzyme, plus AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.

With AccuPrime Taq DNA Polymerase, High Fidelity, you'll get:
• The highest specificity and yield for the most robust PCR amplification
• 9-fold higher fidelity than Taq DNA polymerase alone
• Mimimal optimization steps, even with non-optimized primer sets
• Efficient amplification of targets over a broad size range up to 20 kb

How it works
Pyrococcus species GB-D polymerase is a proofreading enzyme that possesses a 3´→5´ exonuclease activity. Mixing this enzyme with Taq DNA Polymerase increases fidelity and allows amplification of simple and complex DNA templates over a large range of target sizes.

The Platinum antibody complexes with Taq DNA polymerase and inhibits activity at room temperature. Activity is restored after the initial denaturation step, providing an automatic hot start PCR.

The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.

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