规格
|
Label Type:
|
Blocking Groups |
Label or Dye:
|
Unreactive Group |
Product Line:
|
Pierce™ |
Reactive Moiety:
|
Active Ester, Succinimidyl Ester, NHS Ester |
Solubility:
|
DMSO (Dimethylsulfoxide), Water, DMF (Dimethylformamide) |
Spacer:
|
Short |
Chemical Reactivity:
|
Amine |
储存
|
Upon receipt store desiccated at -20° C. Product is shipped at ambient temperature. |
Thermo Scientific Pierce Sulfo-NHS-Acetate is a small compound used for blocking primary amines (e.g., lysine side chains). This compound forms stable, covalent amide bonds with amine groups to prevent specific interactions or conjugations.
Sulfo-NHS-Acetate is typically used to prevent polymerization when performing protein crosslinking reactions and when conjugating peptides to carrier proteins for immunogen production. Blocking amines on the peptide allows directed conjugation of carboxylic acids on the peptide to primary amines on the protein using EDC.
Features of Sulfo-NHS-Acetate:
• Irreversibly block primary amines
• Perform reactions in standard non-amine-containing buffers at pH 7 or greater
• Prevent polymerization when performing protein crosslinking reactions