Human IL-15, premium gr. (100 µg)，130-095-765，美天旎

Recombinant human IL-15 (interleukin 15) promotes proliferation of activated T, NK, and B cells . The regulatory cytokine IL-15 has important diverse roles in the immune system. It is structurally related to IL-2 and they share many functional characteristics. The recombinant protein is optimized for use in cell culture, differentiation studies, and functional assays.

Human IL-15 can be used for a variety of applications, including:

• Activation and expansion of NK and NKT cells.
• In vitro differentiation of NK cells, e.g., from purified CD34⁺ cells.
• In vitro T cell expansion, e.g., of naive CD8⁺ T cells, and T cell activation, e.g., of CTLs.

Biological activity

IL-15 is a member of the four α-helix bundle cytokine family. It is produced by different cell types, including epithelial cells, monocytes, muscle and placenta cells. IL-15 is a potent lymphoid cell growth factor. It stimulates the proliferation of activated T cells and promotes the generation of cytotoxic T lymphocytes (CTLs). IL-15 also induces the generation, proliferation, and activation of NK cells as well as B cell growth and immunoglobulin production. In addition, IL-15 is important for the maintenance of CD8⁺ memory T cells. For binding and signaling IL-15 uses the unique IL-15 receptor α-chain, but shares the β- and γ-chain of the IL-2 receptor.

Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).