规格
|
Product Line:
|
Pierce™ |
Crosslinker Type:
|
Heterobifunctional |
Labeling Method:
|
Chemical Labeling |
Reactive Moiety:
|
Maleimide, Sulfo-NHS Ester |
Solubility:
|
Water |
Spacer:
|
Short |
储存
|
Upon receipt store desiccated at 4° C. Product is shipped at ambient temperature. |
Thermo Scientific Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).
Features of Sulfo-EMCS:
• Reactive groups: sulfo-NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• Sulfo-NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-soluble (compare to EMCS)
• Aliphatic spacer offers low potential for eliciting an immune response
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)