规格
Accession Number:	BC074796.2
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	CHO-K1
Cell State:	Dividing Cells
Druggable Target:	GPCRs
Gene Symbol:	HCRTR1
Population Status:	Pool of Stable Cells
Product Line:	GeneBLAzer®
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	HCRTR1
Detection Method:	Fluorescent

储存
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: o GeneBLAzer ® HCRTR1 -NFAT-bla CHO-K1 cells o GeneBLAzer ® HCRTR1 -NFAT-bla CHO-K1 Assay Protocol o Certificate of Analysis The GeneBLAzer® HCRTR1 -NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

The GeneBLAzer® HCRTR1-NFAT-bla CHO-K1 cells contain the human Hypocretin (Orexin) Receptor 1 (HCRTR1), (Accession # NM_001525) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT Response Element.

The HCRTR1-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of Orexin A. In addition, HCRTR1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

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