规格
Activity:	0.1 ng/ml
Bioassay:	Proliferation of BALB⁄3T3 cells
Endotoxin Level:	< 0.1 ng/µg
Product Line:	CTS™
Purity or Quality Grade:	Carrier-Free
Shipping Condition:	Wet Ice
Expression System:	E. coli
Protein Family:	Growth Factors & Receptors
Protein Form:	Full Length, Recombinant, Ligand
Protein Subtype:	FGFs (Fibroblast Growth Factors)
Research Category:	Clinical Research, Oncology, Angiogenesis, Stem Cell Research, Microbiology, Differentiation, Neurobiology
Species:	Human
Validated Application:	Cell Culture
Molecular Weight:	17.2 kDa
Purification Method:	Sequential Chromatography
Purity:	95 %
Gene Alias:	FGF
Protein Length:	155 aa

储存
2° to 8° C

描述

Cell Therapy Systems CTS™ Recombinant Human FGF-basic (full length) is a bioactive protein intended for use in cell culture applications, including pre-clinical and clinical cell therapy studies. FGF-basic (FGFb), is involved in a number of biological processes including embryonic development and differentiation, and neuron differentiation, survival, and regeneration. FGFb is also involved in the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. The lyophilized, E. coli-expressed FGF-basic (full length) CTS™ Recombinant Human is verified for activity through dose-dependent proliferation of BALB/3T3 cells.

• Highly Pure—minimizes interference from other proteins or contaminants
• Active—get more results with less protein

High Purity
To help verify that GIBCO® CTS™ Recombinant growth factors are of the highest quality, each protein is analyzed for purity, along with structural homogeneity, to help ensure a biologically active protein. The purity of each GIBCO® CTS™ recombinant growth factor is verified by SDS-PAGE to be over 95% pure and to have endotoxin levels below 0.1 ng/μg.

High Biological Activity
All GIBCO® CTS™ Recombinant growth factors are verified for activity. Each lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our FGF-basic (full length) CTS™ Recombinant Human growth factor is determined by the dose-dependent proliferation of BALB/3T3 cells to have an ED50 range of 0.1–1.0 ng/mL (specific activity: 1.0 × 10⁷–1.0 × 10⁶ units/mg). Optimal concentration for individual applications should be determined by a dose response assay.

Related Links
• Learn More About GIBCO® Cell Therapy Systems (CTS™) Products
• Cell Therapy Systems Technical Support Web Page
• Our Full Collection of GIBCO® CTS™ Growth Factors
• Our Full Collection of GIBCO® Growth Factors

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