规格
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Delivery Type:
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Transfection |
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Key Functions:
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Reporter Assays |
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Product Line:
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Pierce™ |
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Reporter Gene:
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TurboLuc™16 Luciferase |
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Cloning Method:
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Restriction Enzyme ⁄ MCS |
储存
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Store in freezer (-5 to -30°C). |
The Thermo Scientific™ pMCS Tluc16 Vector is a multiple cloning site (MCS) vector designed to accept a promoter sequence for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter.
• Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning sites provide versatility for transfer of regulatory elements from one vector to another
• Synthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-through
Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression. A synthetic polyA terminator and a Transcriptional Pause Site (TPS) are included upstream of the MCS to minimize non-specific transcriptional read-through. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.