Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas.

This RPMI is modified as follows:

With	Without
• L-glutamine	• HEPES
• Phenol Red

RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂ and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g ⁄ L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Gibco® 'BASIC' products offer value-minded scientists liquid cell culture media and buffer solutions at a significant price savings. As part of Life Technologies’ commitment to quality, this select range of Research Use Only products is continually tested to ensure performance and customer satisfaction.

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