规格
Cloning Method:	Restriction Enzyme ⁄ MCS
Product Line:	InsectSelect™
Protein Tag Position (to your gene):	C-terminal
Promoter:	OplE2
Vector:	pMIB
Protein Tag:	His Tag (6x), V5 Epitope Tag

储存
The pMIB/V5-His Vector Kit contains 20 µg each of supercoiled, lyophilized pMIB/V5-His A, B, and C vectors, pMIB/V5-His/CAT control vector, and 2 µg each of the OpIE2 Forward and Reverse Sequencing primers. Store at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

描述

pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:

• The OpIE2 promoter for constitutive expression
• The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin

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