规格
|
Cell Permeability:
|
Cell-Permeant |
Excitation/Emission:
|
361⁄497 |
Product Line:
|
Ready Flow™ |
Sub Cellular Localization:
|
Nucleus |
Color:
|
Blue |
储存
|
2 dropper bottles, store at room temperature |
Hoechst 33342 Ready Flow™ Reagent is a bright, easy-to-use cell-permeant stain for cell cycle analysis on a flow cytometer. This dye emits blue fluorescence when bound to double-stranded DNA with an emission maximum at 461 nm.
• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Cell-permeable and effective in fixed and live-cell staining for cell cycle
Hoechst 33342 Ready Flow Reagent is a cell-permeable, blue fluorescent DNA stain for cell cycle analysis. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.
Hoechst 33342 reagent binds preferentially to adenine-thymine regions of double-stranded DNA. This dye binds into the minor grooves of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios. Hoechst 33342 Ready Flow Reagent is excited by UV light and has peak emission at 460 nm.