规格
Species:	P. pastoris
Vector:	pPIC
Promoter:	AOX1
Antibiotic Resistance Bacterial:	Ampicillin (AmpR)
Bacterial or Yeast Strain:	GS115, KM71
Cloning Method:	Restriction Enzyme ⁄ MCS
Expression System:	Yeast
Expression Mechanism:	Cell-Based Expression
Protein Tag:	Untagged

储存

The Original Pichia Expression Kit includes 10 µg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression, Pichia pastoris strains GS115 (his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 µg each 5´ AOX1, 3´ AOX1, and α-factor sequencing primers. Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored.

描述

The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium. The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.

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