规格
Final Product Type:	Plasmid DNA
For Use With (Application):	Next-Generation Sequencing, Cloning, Sequencing, Transformation, Transfection, In Vitro Transcription
Purification Target:	Plasmid DNA
Sample Type:	Bacterial Culture
Isolation Technology:	Spin Column
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Prep Scale:	100-200 µg (Medium-Scale) Plasmid DNA
Product Line:	S.N.A.P. ™

储存
Store the S.N.A.P.™ MidiPrep filtering columns, S.N.A.P.™ MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, and 4X final wash at room temperature. Store the resuspension buffer, precipitation salt, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

描述

The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture (Figure 1). The S.N.A.P.™ MidiPrep procedure takes just 40 minutes. You simply:

• Lyse cells and filter debris with the S.N.A.P.™ filtering column
• Apply flow-through to the S.N.A.P.™ DNA binding column
• Wash the column
• Elute pure, supercoiled plasmid DNA in water or TE buffer

Plasmid DNA purified with the S.N.A.P.™ MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ~200 ng/µl-perfect for subsequent applications including mammalian cell transfection. Typical yields are shown in Table 1.

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