规格
|
Antibody Conjugate:
|
Lanthanide Chelates, Terbium (Tb) |
Antibody Molecular Weight:
|
150000 Da |
Assay Entry:
|
Biochemical terbium phospho-specific activity |
Excitation/Emission:
|
340⁄490, 520 |
Label Type:
|
Lanthanide Chelates |
Label or Dye:
|
Tb (Terbium), GFP (EmGFP) |
Product Line:
|
LanthaScreen® |
Reactivity:
|
Human |
Reactivity abrv:
|
Hu |
Readout:
|
End Point |
Shipping Condition:
|
Dry Ice |
Target Entry:
|
mTOR, FRAP1 |
Target Enzyme:
|
Serine⁄Threonine Kinases |
Technique:
|
TR-FRET |
Validated Kinases:
|
FRAP1 (mTOR), FRAP1 (mTOR) |
Format:
|
384-well plate |
储存
|
Terbium-labeled anti-4E-BP1 [pThr46] antibody in HEPES buffered saline. Store at -20°C. |
A part of the PI3K/AKT Pathway, 4E-BP1 (EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1) is a cytosolic protein that is phosphorylated at Thr46 by mTORC1 upon stimulation of the pathway.
For use in combination with the GFP-4E-BP1 physiological protein substrate (PV4759) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.