规格
|
Detection Method:
|
Fluorescent |
Excitation/Emission:
|
630⁄650 |
For Use With (Equipment):
|
Imaging |
Form:
|
Liquid |
Product Line:
|
CellTracker™ |
Reagent Type:
|
Cell Tracker Compounds |
Label Type:
|
Other Label(s) or Dye(s) |
Label or Dye:
|
CellTracker™ Deep Red |
储存
|
Store in freezer (-5 to -30°C), dessicate, and protect from light. |
CellTracker™ Deep Red is a fluorescent dye well suited for monitoring cell movement or location. After loading the cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The deep red excitation/emission spectra are ideal for multiplexing with green and red fluorescent dyes and proteins.
Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.
• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Red excitation/emission spectra (630/650 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation
The CellTracker™ Deep Red fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell-impermeant reaction products. CellTracker™ Deep Red dye is retained in living cells through several generations. The dye is transferred to daughter cells but not adjacent cells in a population. CellTracker™ Deep Red dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Deep Red dye are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.