规格
Accession Number:	NM_013345
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	FreeStyle™ 293-F Cells
Cell State:	Dividing Cells
Druggable Target:	GPCRs
Gene Symbol:	G2A
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	GPR132, G2A
Technique:	FRET
Detection Method:	Fluorescent

储存

Contents:
K1749 GeneBLAzer® TREx™-G2A-NFAT-bla Freestyle293F cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® TREx™-G2A-NFAT-bla Freestyle293F cells
• GeneBLAzer® TREx™-G2A-NFAT-bla Freestyle293F Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® TREx™-G2A-NFAT-bla Freestyle293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession # NP_037477) stably integrated into the CellSensor® NFAT-bla Freestyle HEK 293F cell line. CellSensor® NFAT-bla Freestyle HEK 293F cells (Cat. no. K1725) contain a beta-lactamase reporter gene under control of the NFAT Response Element.

The GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells are functionally validated for Z' and EC₅₀ concentrations of dDAVP. In addition, GeneBLAzer® GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

G2A is an orphan GPCR. Upon overexpession G2A is constitutively active. This is an assay for the orphan GPCR based upon constitutive activity. To use the assay to detect inverse agonists, high amounts of doxycycline should be added. To use the assay to detect agonists, low amounts of doxycycline should be added.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

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