The Viobility™ Fixable Dyes allow the discrimination between live and apoptotic or dead cells by flow cytometry. The dyes are suitable for both fixed and unfixed cells.

Following reagents are available, addressing different fluorescent channels:

Viobility 405/452 Fixable Dye (Ex.: 405 nm, Em.: 452 nm)
Viobility 405/520 Fixable Dye (Ex.: 405 nm, Em.: 520 nm)
Viobility 488/520 Fixable Dye (Ex.: 488 nm, Em.: 520 nm)
Viobility 640/770 Fixable Dye (Ex.: 640 nm, Em.: 770 nm)

The Viobility™ Fixable Dyes have been developed for the detection and discrimination of dead cells. These dyes react with the primary amine groups of proteins which can be found on the cell surface as well as intracellularly.

In contrast to viable cells, dead cells exhibit a compromised cell membrane allowing the Viobility Fixable Dyes to enter the cell and stain proteins within the cell. This results in a up to 50-fold brighter fluorescent staining and thus allows the discrimination between dead and viable cells by using a flow cytometer.

Viobility Fixable Dyes show certain advantages over traditional dead cell–staining dyes like propidium iodide (PI):

non-toxic to cells
less spillover into other fluorescent channels
more flexibility in antibody panel design, as dyes are available for various excitation and emission wavelengths

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