规格
|
Antibiotic Resistance Bacterial:
|
Ampicillin (AmpR) |
Cleavage:
|
EK (Enterokinase) Recognition Site |
Cloning Method:
|
Gateway® |
Product Line:
|
Gateway® |
Constitutive or Inducible System:
|
Inducible |
Inducing Agent:
|
Arabinose |
Promoter:
|
araBAD |
Selection Agent (Eukaryotic):
|
None |
Vector:
|
pBAD, pDEST |
Protein Tag:
|
His Tag (6x), V5 Epitope Tag, Thioredoxin |
储存
|
The pBAD-DEST49 Gateway® destination vector includes 6 µg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored. |
The pBAD-DEST49 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:
• The araBAD promoter for tightly regulated expression in E. coli
• HP-thioredoxin fusion for improved protein yield and solubility
• Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™
• C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
• R sites for efficient recombination with any attL-flanked Gateway® entry vector