规格
Accession Number:	NM_005161
Assay Entry:	Cell-based beta-arrestin recruitment
Cell Line:	U2OS
Cell State:	Division-Arrested Cells
Cytoplasmic Tail:	Native Tail
Druggable Target:	GPCRs
Gene Symbol:	AGTRL1
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®
Protein Family:	GPCR (G-Protein Coupled Receptors)
Readout:	End Point
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	Tango™
Target Entry:	AGTRL1
Detection Method:	Fluorescent

储存

K1769 Tango™ AGTRL1-bla U2OS DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• Tango™ AGTRL1-bla U2OS DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• Tango™ AGTRL1-bla U2OS DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis
The Tango™ AGTRL1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

The Tango™ AGTRL1-bla U2OS DA cells contain the human Angtiotension-Like Receptor 1 (AGTRL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ AGTRL1-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ AGTRL1-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

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