TRYPLE EXPRESS 5L, BAG，12604054，Invitrogen

规格
Chelators:	EDTA
Classification:	Animal Origin-Free
Enzyme Stability:	Room Temperature Stable
Osmolality:	Check and Record
Product Line:	Gibco®
Reagent Type:	Trypsin Replacement
Shipping Condition:	Room Temperature
Tested For:	In Vitro Bioassay
Form:	Liquid
Phenol Red Indicator:	No Phenol Red
pH Range:	Check and Record
Concentration:	1 X
Quantity:	1 universal bag(s)

储存

Storage conditions: 15°C to 30°C, protect from light Alternate storage conditions: 2°C to 8°C or -5 to -20°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture

描述

TrypLE™ Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety of TrypLE™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE™ Express is:

• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room temperature-stable
TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE™ Express is free of animal-derived components.

cGMP manufacturing and quality system
TrypLE™ Express is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.