规格
Accession Number:	NP_002746, S218D, S222D
Assay Entry:	Biochemical binding/kinetic
Kinase Group:	Ser⁄Thr Kinases: STE Group
Mass:	10 µg
Shipping Condition:	Dry Ice
Tag Position:	N-Terminal
Target Entry:	MAP2K1 S218D S222D
Target Gene:	Mitogen-activated protein kinase kinase 1
Expression System:	Baculovirus
Protein Family:	Kinases & Inhibitors
Protein Form:	Full Length, Recombinant
Protein Subtype:	Serine⁄Threonine Kinases
Research Category:	Signal Transduction
Species:	Human
Validated Application:	Kinase Assay
Gene ID (Entrez):	5604
Molecular Weight:	49.2 kDa
Gene Alias:	MAP2K1 (MEK1) S218D S222D
KinaseFamily:	STE7 Kinase Family
Protein Tag:	His Tag

储存
Storage Buffer: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.05 mM EDTA, 0.05% NP-40, 1mM DTT, 50% glycerol Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.

描述

MAP2K1 (MEK1) kinse is a dual-specific protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. This mutant has been activated by subsituting serines 218 and 222 with aspartic acid to mimic the phosphorylated state.

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