- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Final Product Type: | Total RNA |
| For Use With (Application): | Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting |
| Purification Target: | Total RNA |
| Sample Type: | Tissue, Cells |
| Isolation Technology: | Silica Spin Column |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Product Line: | Ambion™, RNAqueous® |
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储存 |
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• 600 µl 10X DNase I Buffer (-20°C) • 45 µl DNase I 2 U/µl (-20°C) • 1 ml Formaldehyde Load Dye (-20°C) • 100 µl Linear Acrylamide 5 mg/ml (-20°C) • 50 µl Positive Control Primers (-20°C) • 1 ml 5M Ammonium Acetate (-20°C) • 35 ml Water for 64% Ethanol (4°C) • 600 µl DNase Inactivation Reagent (4°C) • 30 ml Lysis/Binding Solution (4°C) • 25 ml Wash Solution 1 (4°C) • 35 ml Wash Solution 2/3 (4°C) • 3 Pestles for tissue disruption (room temperature) • 30 Filter Cartridges (room temperature) • 60 Collection Tubes (room temperature) • 25 ml Elution Solution (-20°C, 4°C, or room temperature) |
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描述 |
The RNAqueous®-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (~100 cells to ~1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. Features of this kit:
• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous®-4PCR Kit
The RNAqueous®-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater® Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
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¥ 749.00
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¥ 2105.00
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