规格
Label or Dye:	Unconjugated
Format:	Tube(s)
Includes:	Control Primers & Template(s)
Gene Symbol:	ACTB
5'Primer Modification:	None
3'Primer Modification:	None
Control Template:	XenoRNA™ control (synthetic RNA transcript)
Shipping Condition:	Dry Ice
Target Gene:	XenoRNA™ control, β-actin
Product Line:	Cells-to-CT™, SYBR®

储存
Components: 100 µL Xeno™ RNA Control, 10^5 copies/µL 250 µL 20X SYBR Xeno Primers 250 µL 20X SYBR ACTB Primers Amounts: Xeno RNA Control: approximately 100 lysis reactions SYBR Xeno and SYBR ACTB Primers: up to 250 PCRs (20 µL) each Storage Conditions: Store at −20°C. Do not store in a frost-free freezer.

描述

This kit is designed for use with the Power SYBR® Green (SKU#s 4402953, 4402954, and 4402955) and Fast SYBR® Green Cells-to-C_T™ Kits (SKU#s #4402956 and 4402957). Sufficient reagents are provided for 100 reactions. It includes Xeno™ RNA Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, and a PCR primer set for the Xeno™ RNA Control target. It also includes a PCR primer set for the highly expressed endogenous control gene β-actin (ACTB). Together these reagents provide positive controls for reverse transcription and SYBR® Green-based real-time PCR, and can indicate the presence of any reverse transcription or PCR inhibitors. Real-time RT-PCR with SYBR® ACTB Primers can also provide an endogenous control for sample normalization, as well as to confirm sufficient cell input in experiments with samples consisting of more than 100 cells per lysis.

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