规格
Antibiotic Resistance Bacterial:	Ampicillin (AmpR)
Bacterial or Yeast Strain:	LMG194, TOP10
Cleavage:	EK (Enterokinase) Recognition Site
Cloning Method:	TOPO®-TA
Expression Mechanism:	Cell-Based Expression
Expression System:	E. coli
Product Line:	TOPO®
Constitutive or Inducible System:	Inducible
Inducing Agent:	Arabinose
Promoter:	araBAD
Selection Agent (Eukaryotic):	None
Quantity:	20 reactions
Vector:	pBAD
Protein Tag:	His Tag (6x), V5 Epitope Tag

储存

Each pBAD TOPO® TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO® TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO® vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot® box contains all of the reagents necessary for transformation, including single-use 50 µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot® cells at -80°C. Store the LMG194 E. coli stab at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.

描述

The pBAD TOPO® TA Expression Kit is specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products. Once you've TOPO® Cloned your PCR product, you can go straight to protein expression. Some of the convenient features of the pBAD-TOPO® vector include:

• Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
• The araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with an Anti-V5 Antibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• Enterokinase cleavage site for removal of N-terminal leader peptide

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