规格
Final Product Type:	Total RNA
For Use With (Application):	Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
For Use With (Equipment):	MultiPROBE® II HT, Biomek® 2000
Purification Target:	Total RNA
Sample Type:	Mammalian Cells, Tissue (Mammalian)
Format:	96-well plate
Isolation Technology:	Vacuum Filter Plate (Glass Fiber Filter)
High-throughput Compatibility:	High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Starting Material Amount:	Up to 1.0 mg tissue, Up to 5 x 10^5 cells
Product Line:	Ambion™, RNAqueous®

储存
Store Filter Plates (4), Collection Plate with lids (4), Culture Plates (4), Heat Plate with lid (1), Nuclease-free Water (100 mL), and Elution Solution (100 mL) at room temperature. Store Lysis⁄Binding Solution (100 mL), Rebinding Mix (100 mL) and Wash Solution Concentrate (2 x 175 mL) at 4°C. Store DNase I (1.25 mL; 20U⁄µL) and DNase 1 Buffer (12 mL) at -20°C.

描述

The Ambion® RNAqueous®-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous®-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource.

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