规格
Assay:	Phosphatase Assay
Detection Method:	Fluorescent
Excitation/Emission:	430⁄450
For Use With (Equipment):	Microplate Reader
Form:	Liquid
Label or Dye:	MDCC
Readout:	Kinetic
Shipping Condition:	Dry Ice
Target Enzyme:	GTPases, ATPases, Phosphatases, Phosphodiesterases
Technique:	Fluorescence Intensity
Format:	384-well plate
Quantity:	10nmol

储存
Phosphate Sensor is supplied as a 500 µM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.

描述

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ~ 0.1 µM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.

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