规格
Final Product Type:	Plasmid DNA
For Use With (Application):	Cloning, PCR
Purification Target:	Plasmid DNA
Sample Type:	Bacterial Culture
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Prep Scale:	< 100 µg (Small-Scale) Plasmid DNA
Shipping Condition:	Room Temperature
Product Line:	CloneChecker™

储存
The CloneChecker™ System includes Yellow, Red, and Green solutions and Loading buffer. Store at 15°C to 30°C.

描述

CloneChecker™ System is a rapid method for screening recombinant bacterial colonies or liquid cultures of colonies for the presence of target plasmid DNA (1). The CloneChecker™ System:

• Screens bacterial colonies for plasmid DNA and insert of interest prior to purification
• Screens for inserts >200 bp in plasmids up to 15 kb by size comparison to the original vector or a DNA size marker
• Goes from plated colonies through lysis in less than 5 minutes
• Allows for supercoiled, restriction digest, or PCR analysis
• Works with single-copy plasmids starting with a 2-mm bacterial colony
• Includes sufficient reagents for processing 100 bacterial samples by supercoiled analysis (direct size comparison); and 100 samples by restriction endonuclease or PCR analysis

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