规格
|
Assay Entry:
|
Biochemical terbium phospho-specific activity |
Detection Method:
|
Fluorescent |
Gene ID (Entrez):
|
9833 |
Label or Dye:
|
GFP (EmGFP) |
Shipping Condition:
|
Dry Ice |
Substrate:
|
EIF2A |
Substrate Properties:
|
Protein-Based Substrate |
Target Entry:
|
EIF2AK1, EIF2AK2, EIF2AK3 |
Target Enzyme:
|
Serine⁄Threonine Kinases |
Validated Kinases:
|
EIF2AK2, EIF2AK3 |
Substrate Type:
|
Kinase Substrate |
Molecular Weight:
|
69100 Da |
储存
|
GFP-EIF2alpha protein substrate in 9 mM HEPES pH 7.5, 123 mM NaCl, 2.43 mM KCl, 10% Glycerol, 2mM DTT . Store at -80 °C. |
EIF2A has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the EIF2AK family of kinases including PERK (EIF2AK3) and PKR (EIF2AK2).
For use in combination with the LanthaScreen® Tb-anti-eIF2alpha [pSer52] Antibody (PV4810) to evaluate inhibitors in medium to high throughput screening applications.