规格
Cloning Method:	Gateway®
Expression System:	Mammalian
For Use With:	MultiSite Gateway® Pro kits (e.g. cat. no. 12537-100)
Key Functions:	Clone your own Promoter, Multi-Gene Expression
Product Line:	Gateway®, pcDNA™
Protein Tag Position (to your gene):	C-terminal
Delivery Type:	Transfection
Promoter:	None (Promoterless)
Vector:	pcDNA, pDEST, MultiSite Gateway Vectors
Protein Tag:	V5 Epitope Tag

储存
All destination vectors are provided lyophilized and supercoiled.

描述

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-pL-DEST vector offers the following key features:

•Promoterless version of the pcDNA™6.2⁄V5-DEST vector (cat. no. 12489027)
•attR sites for Gateway® cloning
•Compatible with MultiSite Gateway® Pro kits (e.g. cat. no. 12537100)
•C-terminal V5 tag for easy detection
•Blasticidin resistance gene for efficient stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway® Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway® entry clone, appropriate Gateway® LR Clonase® enzyme mix, and reaction buffer.

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