规格
Bacterial or Yeast Strain:	DH5α
Blue/White Screening:	Yes
Cloning Methylated DNA:	No
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
Competent Cell Type:	Chemically Competent
F'episome (to make ssDNA):	Lacks F' Episome
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Line:	DH5a, MAX Efficiency™
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	Yes
Shipping Condition:	Dry Ice
T1 Phage - Resistant (tonA):	No
Transformation Efficiency:	> 1x10^9
Transformation Efficiency Level:	High Efficiency (> 10^9 cfu⁄µg)
Format:	Tube(s)
Improves Plasmid Quality:	Yes
Species:	E. coli

储存
Contains: • Max Efficiency DH5α Competent Cells: 5 vials, 200 µl each (total of 1 ml) • pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl • S.O.C. medium: 2 bottles, 6 ml each Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

描述

MAX Efficiency DH5α Competent Cells are a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Features of MAX Efficiency DH5α Competent Cells include:

• Transformation efficiency up to 1 × 10⁹ transformants/µg plasmid DNA
• High plasmid yield from the DH5α (endA1) E. coli strain
• Blue/white screening capable (lacZΔM15)
• Greater insert stability (recA1)

About MAX Efficiency DH5α Competent Cells
MAX Efficiency DH5α Competent Cells have been prepared by a proprietary modification of the procedure of Hanahan (1983). These cells are suitable for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can therefore be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn of DH5α-FT, DH5αF′, DH5αF′IQ, JM101, or JM107 is provided to allow plaque formation.

Genotype: F^- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk^-, mk⁺) phoA supE44 λ^-thi^-1 gyrA96 relA1

Find the strain and format that you need
DH5α cells are available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats and in single-use aliquots.

• Subcloning Efficiency DH5α Competent Cells—our most economical competent cell for everyday use
• Library Efficiency DH5α Competent Cells—economical and for use with difficult-to-transform DNA
• MAX Efficiency DH5α-T1^R Competent Cells—T1 phage resistant, >10⁹ transformants/µg plasmid DNA
• One Shot MAX Efficiency DH5α-T1^R Competent Cells—single-use format, T1 phage resistant, >10⁹ transformants/µg plasmid DNA
• ElectroMAX DH5α Competent Cells—electrocompetent with >10¹⁰ transformants/µg plasmid DNA and ideal for cDNA libraries or larger inserts

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