RAS TOTAL ABS QUAN MODULE 10 RXNS，A40098，赛默飞世尔

The Thermo Scientific SureQuant RAS Isoform Absolute Quantitation Module provides a complete quantitation solution for the analysis of key RAS expression using mass spectrometry (MS).

SureQuant RAS Isoform Absolute Quantitation Module features include:
• Complete—contains validated AQUA HeavyPeptide and LightPeptide mixtures, diluent, matrix, and system suitability standard including optimized acquisition and analysis methods
• Validated—peptides rigorously tested for successful quantitation of each target peptide
• Multiplex—able to quantitate NRAS, KRAS, and HRAS isoforms

The RAS family of GTPase proteins modulates two key signaling pathways involved in cell growth and proliferation: the MAPK and PI3K pathways. Aberrant activation of the RAS proteins promotes constitutive activation of cell growth and proliferation that can result in cancer. Mutations in the NRAS, KRAS, and HRAS oncogenes are the most common mutations in human cancer. This kit is ideal for monitoring expression levels of the three isoforms in a single assay. The ability to simultaneously monitor and quantitate in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant RAS Isoform Absolute Quantitation Module provides reagents for absolute quantitation of three unique peptides from RAS using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mix, AQUA Ultimate LightPeptide mix, and Pierce 6 Protein Digest, equimolar, LC-MS grade reagents are used to generate a standard curve in a matrix, and serial dilutions performed by the user have been optimized to span 2–3 orders of magnitude (200 to 0.03 fmol on column). Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

This kit can be used with SureQuant RAS Isoform IP and MS Sample Preparation Module for enriched multiplex sample preparation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

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