规格
Cloning Method:	TOPO®-TA
Key Functions:	Reporter Assays, Clone your own Promoter
Product Line:	TOPO®
Constitutive or Inducible System:	Constitutive
Delivery Type:	Transfection
Promoter:	None (Promoterless)
Reporter Gene:	Beta-Gal (lacZ)
Selection Agent (Eukaryotic):	Geneticin® (G-418)
Vector:	pBlue, TOPO-TA Vectors
Protein Tag:	Untagged

储存

pBlue TOPO® TA Expression Kits contains two boxes. The TOPO® TA Cloning box contains linearized and topoisomerase I-activated pBlue-TOPO® vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers. This box should be stored at -20°C. The One Shot® box contains One Shot® TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.

描述

The pBlue TOPO® TA Expression Kit uses β-galactosidase expression to assay a wide range of transcriptional activity from promoter fragments. Assays for β-galactosidase are easy to perform and are quantitative at very low levels of expression. The pBlue TOPO® TA Expression Kit is ideal for functional analysis of promoters with low transcriptional activity.

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