规格
Cloning Method:	Gateway®
Key Functions:	Reporter Assays, Subcellular Localization
Product Line:	Gateway®, Vivid Colors™, pcDNA™
Constitutive or Inducible System:	Constitutive
Delivery Type:	Transfection
Promoter:	CMV
Reporter Gene:	YFP
Selection Agent (Eukaryotic):	Blasticidin
Vector:	pDEST, pcDNA
Protein Tag:	V5 Epitope Tag, YFP

储存
Each vector is supplied as supercoiled and lyophilized plasmid DNA. Store at -20°C after resuspended. Guaranteed stable for 6 months when properly stored.

描述

The Vivid Colors™ Fluorescent Protein Gateway® Destination Vectors allow you to quickly and easily fuse a protein of interest to the widely used and well-characterized fluorescent proteins from the jellyfish Aequorea victoria (1,2) using the pcDNA™ 6.2 Gateway™ mammalian expression vector. These powerful Gateway® Technology vectors contain the next-generation EGFP, Emerald Green Fluorescent Protein (EmGFP), or the popular Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein. Both fluorescent proteins have been humanized for optimal mammalian expression (3). In addition, the Vivid Colors™ pcDNA™ 6.2 Fluorescent Protein Gateway® Vectors offer:

• CMV promoter for high-level expression of the recombinant fluorescent fusion protein
• Options to fuse EmGFP or YFP to the N- and C-terminus of your protein

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