规格
Final Product Type:	DNA
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Isolation Technology:	Silica Spin Column
No. of Reactions:	20 Reactions
Product Line:	Ambion™, DNAclear™
Purification Target:	DNA from Enzymatic Reactions (e.g. PCR)
Purification Time:	10 min
Sample Type:	PCR Products
Scale:	Mini
Shipping Condition:	Room Temperature
Starting Material Amount:	1 ng to 3 µg
Yield:	3 µg
Format:	6 microtubes
For Use With (Application):	PCR, Sequencing, In Vitro Transcription, Cloning
For Use With (Equipment):	Centrifuge

储存
cDNA Binding Buffer, cDNA Wash Buffer, Microfilter Cartridges + Collection Tubes, and Elution Tubes should be stored at room temperature. Nuclease-free water may be stored at any temperature.

描述

The Ambion® DNAclear™ kit is designed to purify double-stranded DNA (dsDNA) generated by enzymatic reactions such as cDNA synthesis and PCR. Includes sufficient reagents for 20 purifications.

• Efficiently purify and concentrate dsDNA ranging from 1 ng to 3 µg
• Ideal for downstream applications such as in vitro transcription
• Simple and fast protocol—about 10 minutes

The DNAclear™ cDNA Purification Kit process is simple and fast. DNA amounts ranging from 1 ng to 3 µg can be efficiently purified and concentrated. The DNAclear™ procedure consists of three steps: 1) DNA is bound to the Microfilter Cartridge in the presence of the DNA Binding Buffer, 2) the DNA is washed with the cDNA Wash Buffer, and 3) the DNA is eluted with preheated nuclease-free water. The DNAclear™ Kit can be used to remove free nucleotides, oligonucleotides, proteins, and salts from dsDNA samples. The DNA that is recovered from the Microfilter Cartridge is ready for any application that requires highly pure DNA including in vitro transcription reactions.

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