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StraightFrom® Whole Blood REAlease® CD8 MicroBead Kit, human,130-132-983,美天旎

销售价: ¥ 9361.99 / 件
市场价: ¥ 11014.11 / 件
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订货号 8ND2283
品牌型号 美天旎 130-132-983
货期 询货期
最小订货量 1件
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产品介绍 Product Description

StraightFrom® Whole Blood REAlease® CD8 MicroBead Kit was developed for rapid positive selection of CD8+ cells directly from whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

The purified target cells are bead-free, practical for consecutive magnetic isolations, and can be label-free so that epitopes are available for any downstream application.


Detailed separation procedure

CD8+ cells are labelled with StraightFrom Whole Blood REAlease CD8-Biotin (REAlease Biotin Complex). Subsequently, StraightFrom Whole Blood REAlease Anti-Biotin MicroBeads (CD8, human) bind to REAlease Biotin Complex. Then, the cell suspension is loaded onto a MACS Column, which is placed in the magnetic field of a MidiMACS or QuadroMACS separator. Using the REAlease Bead Release Reagent, which removes the MicroBeads from the cell, target cells are eluted within the magnetic field. Finally, during the subsequent incubation with the REAlease Release Reagent, the REAlease Biotin Complex monomerizes and dissociates from the cell surface leaving the target cells free of all labels.


Downstream applications

StraightFrom Whole Blood REAlease CD8 MicroBead Kit allows the fast isolation of CD8+ cells directly from blood thus minimizing hands-on time and maximizing yields of target cells. The purified CD8+ cells are well-suited for further flow cytometric, functional, or molecular analysis.The isolated CD8+ cell subset is label-free.


Columns

Whole Blood columns, Multi-24 Whole Blood Column Block



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