- 商品介绍
- 规格参数
- 包装参数
规格 |
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For Use With (Application): | Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription |
Isolation Technology: | Anion Exchange Resin, Filter, Vacuum |
Sample Type: | Bacterial Culture |
High-throughput Compatibility: | Manual Protocols |
Prep Scale: | 12 mg plasmid DNA |
Purification Target: | Plasmid DNA |
Shipping Condition: | Approved for shipment at Room Temperature or on Wet Ice |
Product Line: | PureLink™ |
储存 |
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• Equilibration Buffer • Resuspension Buffer • Lysis Buffer • Precipitation Buffer • RNase A • Wash Buffer • Elution Buffer • TE Buffer • Expi HiPure Giga Filtration Column • Expi HiPure Giga DNA Binding Column Store all components at room temperature upon receipt. |
描述 |
The PureLink™ HiPure Expi Plasmid Gigaprep Kit enables isolation of the highest levels of transfection-grade plasmid DNA from a single E.coli giga prep using an enhanced anion exchange resin. The PureLink™ HiPure Plasmid Gigaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times. The PureLink™ HiPure Expi Plasmid Gigaprep Kit typically isolates 12 mg of high quality, ultrapure plasmid DNA with inherently low endotoxin levels from 2.5 L of bacterial culture.
Advantages of the PureLink™ HiPure Expi Plasmid Gigaprep Kit include:
• High yield—isolate up to 14 mg of high quality plasmid DNA from a single purification using 5 L of bacterial culture volume
• Purity—inherently low endotoxin levels (typically 0.1–1.0 EU/µg) and A260/280 >1.8, making it ideal for mammalian cell transfection
• Simple and super-fast protocol—with vacuum assisted protocol, typical plasmid isolation in as little as 90 minutes
HiPure Expi anion exchange chromatography for plasmid purification
PureLink™ HiPure Expi plasmid purification kits use a novel, proprietary anion-exchange resin to enable the purification of the highest levels of transfection-grade plasmid DNA. The enhanced resin combines large capacity with fast flow rate, high resolution, and efficient endotoxin removal. Plasmid preparations are typically completed in less than 2 hours with purity equal to two CsCl gradients. PureLink™ HiPure Expi plasmid purification kits enable the quickest process for obtaining the highest amount of transfection-grade plasmid DNA.
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