规格
Antibiotic Resistance Bacterial:	Ampicillin (AmpR)
Cleavage:	TEV Protease Recognition Site
Cloning Method:	Gateway®
Product Line:	Expressway™, Gateway®
Promoter:	T7
Selection Agent (Eukaryotic):	None
Vector:	pEXP, pDEST
Protein Tag:	Lumio

储存

The Expressway™ Lumio™ Expression and Detection System includes the E. coli slyD- Extract, 2.5X IVPS E. coli Reaction Buffer, T7 Enzyme Mix, the Feed Buffer, 19 amino acid mix, Methionine, a control plasmid, DNAse/RNAse-free Water, and a Lumio™ Green Detection Kit for 20 real-time reactions or 100 in-gel applications. In addition to these components, the N-term Expressway™ Lumio™ kit also includes 6 µg of the pEXP3-DEST vector and the C-term Expressway™ Lumio™ kit 6 µg of the pEXP4-DEST vector. Store the vectors and the detection reagent at -20°C. Store all other components at -80°C. Guaranteed stable for 6 months when properly stored.

描述

The Expressway™ Lumio™ Expression and Detection System takes advantage of the Lumio™ recognition sequence, a small, six amino acid sequence (Cys-Cys-Pro-Gly-Cys-Cys). The Lumio™ detection reagent binds the recognition sequence with high specificity and affinity, resulting in a bright fluorescent signal for real-time protein production analysis and immediate in-gel protein detection. In addition, Expressway’s specialized E. coli lysate, derived from a slyD mutant, eliminates nonspecific binding of the Lumio™ Green Detection Reagent to the endogenous SlyD protein and provides optimal background for detection of recombinant proteins (Figure 1). The Lumio™ Green Detection Kit is included in the system for real-time detection of protein synthesis as well as easy in-gel protein detection. The Lumio™ vectors (Figures 2 and 3) also feature:

• attR sites for efficient recombination with any attL-flanked Gateway® entry vector
• N-terminal Lumio™ tag (pEXP3-DEST vector) with TEV cleavage site for efficient removal of the Lumio™ sequence following purification
• C-terminal Lumio™ tag (pEXP4-DEST vector) for easy, and immediate in-gel detection
• T7 promoter, ribosome binding site, and T7 terminator optimally spaced for cell-free protein expression

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