Mouse IFN-α, research grade，130-134-222，美天旎

IFN-α stands for interferon alpha. Mouse IFN-α is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Mouse IFN-α can be used for a variety of applications including:

• In vitro investigation of antitumor effects, such as antiproliferative capacities on tumor cell lines.
• Studies on signal transduction and gene expression on cell lines.
• In vivo investigation of anti-tumor effects in mouse models of disease.
• Studies of transgenic mice harboring the Cre-recombinase transgene driven by the interferon-inducible Mx promoter (Mx-Cre mice).

Biological activity

Type I interferons (IFNs), including IFN-α, are a family of cytokines that exert multiple functions in the immune system. The most prominent effect of IFN-α is its antiviral activity. Upon viral infections, host cells release IFN-α, which can act in an autocrine or paracrine manner to activate intracellular antiviral defense mechanisms and restrict viral replication. Furthermore, IFN-α affects the generation and function of various dendritic cell populations. Immunomodulatory activity and antitumor effects have been described both in vivo and in vitro.

Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).