The GripTite™ 293 MSR Cell Line is provided as two tubes of a frozen stock of 1 x 10⁷ cells, each in 1 ml of 90% complete media and 10% DMSO. Store in liquid nitrogen. Liquid Geneticin® Selective Antibiotic (20 ml) is supplied as a 50 mg/ml solution. Store at -20°C or 2°C to 8°C. Versene (100 ml) contains 0.2 g/L EDTA 4Na in phosphate-buffered saline. 1X concentration is 0.2 g/L=0.53 mM. Store at 2°C to 8°C. Guaranteed stable for 6 months when properly stored.

描述

The GripTite™ 293 MSR Cell Line is a genetically engineered Human Embryonic Kidney (HEK 293) cell line that expresses the human macrophage scavenger receptor and strongly adheres to standard tissue culture plates for dependable results. Developed from a 293-H subclone, GripTite™ 293 MSR cells show the same fast cell growth, high transfection efficiency, and high-level expression characteristics of the parental 293-H cells. Unlike most 293 cells, GripTite® 293 MSR cells dont wash away during the repeated manipulations of routine tissue culture or plate washing protocols (Figure 1). Their superior adherence enables reliable reproduction of ligand-binding, enzymatic, or immunofluorescence assay results.

Use in High-Throughput Applications
The GripTite™ 293 MSR cells enhance performance in high-throughput cell-based, as well as standard tissue culture, applications. Uncontrolled cell loss during high-throughput protocols leads to unreliable results and expensive, time-consuming repeat experiments. Since GripTite™ 293 MSR cells adhere to standard tissue culture treated plastic, theres no need for the costly poly-lysine coated plates typically used in high-throughput analysis. The GripTite™ 293 MSR cells withstand automated plate washing and protocols using liquid handling robots on standard tissue culture plates without significant cell loss (Figure 2).

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