规格
|
Compatible Cells:
|
Mammalian Cells, Eukaryotic Cells |
Product Line:
|
LIVE/DEAD® |
Shipping Condition:
|
Room Temperature |
Detection Method:
|
Fluorescent |
For Use With (Equipment):
|
Fluorescence Microscope, Flow Cytometer |
Label Type:
|
Other Label(s) or Dye(s) |
Format:
|
Tube(s), Slide(s) |
储存
|
Store in refrigerator (2–8°C) and protect from light. |
The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are preincubated with the green-fluorescent membrane stain DiOC18 and then mixed with effector cells in the presence of the red-fluorescent, membrane-impermeant dye, propidium iodide. Live and dead target cells retain the green-fluorescent membrane stain; target and effector cells with compromised membranes exhibit red-fluorescent nucleic acid staining; live effector cells are nonfluorescent.