规格
Final Product Type:	Transcriptome RNA
For Use With (Application):	Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Isolation Technology:	Magnetic Bead
Sample Type:	RiboMinus™ RNA
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Purification Target:	Transcriptome RNA
Shipping Condition:	Room Temperature
Product Line:	RiboMinus™

储存
Store at room temperature.

描述

The RiboMinus Concentration Module is used to concentrate RiboMinus RNA while retaining species of <200 nucleotides (nt) for further use in downstream applications.

Generally, RNA species of <200 nt are excluded from silica spin columns under standard binding conditions for RNA isolation. Studies have shown the importance of these small RNA species, which include regulatory RNA molecules such as microRNA (miRNA), short interfering RNA (siRNA), and snRNA. To retain RNA of <200 nt during the concentration step of RiboMinus RNA isolation, the RiboMinus Concentration Module is used with a modified silica spin column protocol (binding of RNA is performed with 60% ethanol).

The binding conditions of the spin column protocol are optimized for the RiboMinus RNA sample with ethanol and buffer. The sample is loaded onto a spin column. The RiboMinus RNA binds to the silica-based membrane in the column and impurities are removed by thorough washing with Wash Buffer. The RiboMinus RNA is then eluted in RNase-free water.

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