Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With	Without
L-glutamine	Phenol Red
Proline	Gentamycin
Sodium Pyruvate	HEPES
Zinc	Insulin

The complete formulation is available.

Gibco® Richter's MEM is a modified formulation of Eagle's MEM that contains iron, zinc, putrescine, fatty acids, and gentamycin. Richter's modification also includes very high concentrations of both choline chloride and i-Inositol. This product was further modified with L-glutamine, without gentamicin sulfate, and without phenol red.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

Dual-Site cGMP Manufacturing
Gibco® Richter's MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gibco® MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

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